electrophoresis

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electrophoresis
electrophoresis (ɪˌlɛktrəʊfəˈriːsɪs) [f. electro- + Gr. ϕόρησις being carried.] The migration of colloidal particles suspended in a liquid under the influence of an electric field (cf. cataphoresis). Hence eˌlectrophoˈretic a., eˌlectrophoˈretically adv.1911 Chem. Abstr. 641 Electrophoresis of Lampb... Oxford English Dictionary
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Electrophoresis
Electrophoresis of positively charged particles (cations) is sometimes called cataphoresis, while electrophoresis of negatively charged particles (anions However, Debye length must be important for electrophoresis, as follows immediately from Figure 2, "Illustration of electrophoresis retardation". wikipedia.org
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Gel electrophoresis (article) | Khan Academy
Gel electrophoresis is a technique used to separate DNA fragments (or other macromolecules, such as RNA and proteins) based on their size and charge. Electrophoresis involves running a current through a gel containing the molecules of interest. Based on their size and charge, the molecules will travel through the gel in different directions or at different speeds, allowing them to be separated ...
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Electrophoresis (journal)
Electrophoresis is a peer-reviewed scientific journal covering all aspects of electrophoresis, including new or improved analytical and preparative methods References External links Electrophoresis Biochemistry journals Electrochemistry journals English-language journals Academic journals established wikipedia.org
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Procedure of diagonal electrophoresis I am learning protein sequencing and it seems that diagonal electrophoresis is a common method in the identification of disulphide bonds (S-S) exist between polypeptides in a prot...
You probably don’t _need_ to oxidize the disulfide bonds rather than reduce them. Performic acid has some potential advantages: * The oxidation products can’t spontaneously reform a disulfide bond whereas the reduction products from a thiolate reductant can. * Oxidation by performic acid causes a si...
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Hemoglobin electrophoresis
Procedure The test uses the principles of gel electrophoresis to separate out the various types of hemoglobin and is a type of native gel electrophoresis Newer alternatives to conventional hemoglobin electrophoresis include isoelectric focusing, capillary zone electrophoresis, and high-performance liquid wikipedia.org
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Affinity electrophoresis
Cross electrophoresis, the first affinity electrophoresis method, was created by Nakamura et al. The methods include the so-called electrophoretic mobility shift assay, charge shift electrophoresis and affinity capillary electrophoresis. wikipedia.org
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Is AT or GC content important in electrophoresis? Will it make a difference in running speed if we run samples of same no of bases but different AT - GC content ?
It does make a difference on polyacrylamide. A and C are faster while G and T are slower. Image from publication: !enter image description here The "standards" are AAA/TTT/GGG/CCC molecules.
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Pump to separate protein/DNA instead of electrophoresis? I know that DNA molecules or proteins can be separated in electrophoresis because the electrical charge is used to pull the DNA through the gel. So instead of u...
I am old enough to remember running vertical agarose gel electrophoresis overnight, and these gels would collapse under normal pressure to about 2/3rds
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Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE ... - JoVE
尿素页面或变性尿素聚丙烯酰胺凝胶电泳采用6-8 m尿素,变性中学的dna或rna的结构,是他们根据分子量聚丙烯酰胺凝胶矩阵分离。 2至500个碱基之间的片段,如单核苷酸长度的差异,可以分开使用 此 方法1。样品的迁移是依赖于所选择的丙烯酰胺浓度。
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The Application of Two-dimensional Electrophoresis Technology in ...
As life sciences into post-genome era, proteomics is becoming the new research hot spot. Two-dimensional electrophoresis technology, which has the advantages of high sensitivity, high resolution ...
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2-D Fluorescence Difference Gel Electrophoresis (DIGE) in ...
4.2. TWO-DIMENSIONAL GEL ELECTROPHORESIS. Two-dimensional electrophoresis is one of the most commonly used techniques in proteomics. The basic principles of two-dimensional gel electrophoresis (2D-GE) remain the same since its introduction in 1975 (3,4), namely, the initial separation of proteins by isoelectric focusing (IEF, first dimension) followed by an orthogonal separation via sodium ...
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乙酸钠 suitable for electrophoresis, ≥99%, crystalline | Sigma-Aldrich
乙酸钠 suitable for electrophoresis, ≥99%, crystalline; CAS Number: 127-09-3; EC Number: 204-823-8; Synonyms: 乙酸 钠盐; Linear Formula: CH3COONa; find Sigma-Aldrich-S3272 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich
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