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transcriptase

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transcriptase
transcriptase Biochem. (trɑːnˈskrɪpteɪz, træn-) [f. transcription + -ase.] The polymerase responsible for transcription, which catalyses the formation of RNA from a DNA template; reverse transcriptase, the polymerase responsible for reverse transcription, which catalyses the formation of DNA from an... Oxford English Dictionary
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Telomerase reverse transcriptase
Telomerase reverse transcriptase (abbreviated to TERT, or hTERT in humans) is a catalytic subunit of the enzyme telomerase, which, together with the telomerase The enzyme consists of a protein component with reverse transcriptase activity, encoded by this gene, and an RNA component that serves as a template for wikipedia.org
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Reverse transcriptase (RT)-PCR: Principles, Applications
Feb 21, 2024Reverse Transcriptase PCR (RT-PCR) is a variation of the polymerase chain reaction that amplifies target RNA. Addition of reverse transcriptase (RT) enzyme prior to PCR makes it possible to amplify and detect RNA targets. Reverse transcriptase enzyme transcribes the template RNA and forms complementary DNA (cDNA).
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Non-radioactive nucleotide labeling compatible with reverse transcriptase? Are there any methods for nucleotide labeling which are compatible with use for reverse-transcription, besides radiolabeling, and for which th...
Biotin-labeled nucleotides can be incorporated into cDNA by reverse transcriptase. Jena Bioscience, a provider of biotin-labeled nucleotides, has a table showing which nucleotide chemistries have been successfully used with reverse transcriptase
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Racivir
Racivir is an experimental nucleoside reverse transcriptase inhibitor (NRTI), developed by Pharmasset for the treatment of HIV. References Nucleoside analog reverse transcriptase inhibitors Pyrimidones Oxathiolanes Organofluorides Experimental drugs Hydroxymethyl compounds wikipedia.org
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Discovery and development of nucleoside and nucleotide reverse-transcriptase inhibitors
The HIV-1 reverse transcriptase enzyme Function Most standard HIV drug therapies revolve around inhibiting the reverse transcriptase enzyme (RT), an inhibitor Protease inhibitor Entry inhibitor References Nucleoside analog reverse transcriptase inhibitors Nucleoside And Nucleotide Reverse-transcriptase wikipedia.org
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retravirus
retravirus Biol. (ˈrɛtrəvaɪərəs) [mod.L., f. initial letters of reverse transcriptase (see transcriptase) + virus.] = retrovirus.[1974 Intervirol. IV. 202 Retraviridae are enveloped virions about 100 nm in diameter... They contain about 1.5% of 60–70S RNA... Some species that have been examined also... Oxford English Dictionary
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Discovery and development of non-nucleoside reverse-transcriptase inhibitors
reverse-transcriptase inhibitors (NNRTIs). Non-Nucleoside Reverse Transcriptase Inhibitors, Discovery And Development Of Non-nucleoside reverse transcriptase inhibitors wikipedia.org
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qrt-pcr and short fragments I plan to measure the effects of Tenofovir a Nucleotide analog reverse-transcriptase inhibitors useing qrt-pcr with HIV-RT as the rt enzyme. Tenofovir causes early termination of the revers...
You have to design your primers properly. Usually, in real-time PCR, you don't choose a very long product. Ideal product size is 150-300. Next, see what your NRTI is analogous to. For e.g. if I am using AZT (Azathymidie), I would place my reverse primer at or after the last `T`. There are alternate ...
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Elvucitabine
Elvucitabine belongs to a class (group) of HIV drugs called nucleoside reverse transcriptase inhibitors (NRTIs). By blocking reverse transcriptase, NRTIs prevent HIV from multiplying and can reduce the amount of HIV in the body. wikipedia.org
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non-nucleoside
non-nucleoside, a. and n. Biochem. and Pharmacol. Brit. /nɒnˈnjuːklɪə(ʊ)ˌsʌɪd/, U.S. /nɑ(n)ˈn(j)ukliəˌsaɪd/ [‹ non- prefix + nucleoside n.] A. adj. That is not a nucleoside; spec. designating drugs that inhibit retroviral reverse transcriptase but are not nucleoside analogues, used in the treatment ... Oxford English Dictionary
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SuperScript™ III Reverse Transcriptase - Thermo Fisher Scientific
Features of SuperScript III Reverse Transcriptase include: • First-strand cDNA may be synthesized at temperatures up to 55°C. • cDNA can be generated from 100 bp to >12 kb. • Increased specificity. • High yields of cDNA and full-length products. Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase ...
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Reverse Transcriptase & cDNA Overview & Applications ... | 健康跟著走
Nov 4, 2023reverse transcriptase - Innature,thereversetranscriptaseenzymeiswhatallowsretroviruses(RNAtypevirus)toduplicateandintegratetheirRNAgenomesin...
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Multicopy single-stranded DNA
Tests confirmed the presence of reverse transcriptase activity in crude lysates of retron-containing strains. Retrons The discovery of msDNA has led to broader questions regarding where reverse transcriptase originated, as genes encoding for reverse transcriptase wikipedia.org
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How to clone and sequence a gene transcript of unknown sequence? How might I go about amplifying a gene transcript (mRNA) from animal tissue of which little is known about the genome? In some applications, I have used...
Perhaps you can draw inspiration from classic paper on lambda cloning: **Maniatis T, Hardison RC, Lacy E, Lauer J, O’Connell C, Quon D, Sim GK, Efstratiadis A**. 1978. The isolation of structural genes from libraries of eucaryotic DNA. Cell 15: 687–701. Try selecting tissues from the animal which yo...
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