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supernatant

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supernatant
supernatant, a. and n. (s(j)uːpəˈneɪtənt) [ad. L. supernatant-, -ans, pr. pple. of supernatāre: see super- 2 and natant.] A. adj. a. Swimming above, floating on the surface (as a lighter liquid on a heavier).1661 Boyle Certain Physiol. Ess. (1669) 244 Whilst the substance continu'd fluid, I could sh... Oxford English Dictionary
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supernate
▪ I. † superˈnate, v. Obs. rare. [ad. L. supernatāre: see supernatant.] intr. To float on the surface. b. trans. To float upon or above.1683 Salmon Doron Med. i. xxv. 291 Upon which affuse so much..wine as may supernate them at least four Inches. 1694 ― Bate's Dispens. (1713) 491/2 Separate the supe... Oxford English Dictionary
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Secretomics
Cancer cell line supernatant is an attractive source of secreted proteins. There are many standardized cell lines available and supernatant is much simpler to analyze than proximal body fluid. wikipedia.org
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S9 fraction
National Library of Medicine's "IUPAC Glossary of Terms Used in Toxicology" as the "Supernatant fraction obtained from an organ (usually liver) homogenate wikipedia.org
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Correlating beta-lactamase expression to absorbance > When penicillin-resistant bacteria are grown in liquid culture media, β-lactamase is secreted into the medium. The supernatant of such a medium can be assayed for ...
So higher absorption means more β-lactamase in the supernatant.
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Alkali denaturation test
The pink hemoglobin-containing supernatant is then mixed with 1 mL of 1% NaOH for each 5 mL of supernatant. wikipedia.org
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DNA extraction from agarose gel Because DNA is soluble in water, is it possible to extract PCR product by dissolving excised gel containing the DNA band of desired length in water, mashing the gel piece with a pestle,...
Take the supernatant, do a ethanol precipitation (with glycogen when you only have little DNA) and you have nice and clean DNA.
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Alkaline lysis
The supernatant is discarded, and the pellet is then re-suspended in an EDTA-containing physiological buffer. The plasmid-containing supernatant is carefully removed and can be further purified or used for analysis, such as gel electrophoresis. wikipedia.org
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Iodixanol
Post centrifugation the supernatant above and the cushion below is removed, leaving a concentrated sperm pellet in the conical tube. wikipedia.org
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Does Goryaev's chamber have the same grid layout as the conventional counting chamber used in the West? I'm translating a Russian biotec text where some cell counting operations are mentioned. The piece of equipment t...
From the description at < It appears to be functionally the same device, except the only the smallest square are marked (0.25 nL and 4 nL), and the total capacity is 90 nL instead of 100 nL. A hemocytometer could be used in place of a Goryaev's chamber (though not necessarily the reverse).
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NUN buffer
By incubating nuclei in NUN buffer and centrifuging the solution, the supernatant will therefore contain nuclear proteins. wikipedia.org
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Concentration of DNA by isopropanol I have read that DNA can be concentrated by addition of isopropanol. What does "concentrated" mean? What does isopropanol do on a molecular level to concentrate DNA?
Finally the solution is centrifuged to collect the DNA at the bottom of your reaction tube and to be able to take off the supernatant.
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Hybridoma technology
This maintains the well-being of the hybridomas and provides enough cells for cryopreservation and supernatant for subsequent investigations. The culture supernatant can yield 1 to 60 μg/ml of monoclonal antibody, which is maintained at -20 °C or lower until required. wikipedia.org
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Sludge bulking
Sludge is continually drawn off as new sewage enters the tank and this sludge must then be settled so that the supernatant (the remaining liquid) can be wikipedia.org
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Protein L
requirements for effective binding, the main application for immobilized Protein L is purification of monoclonal antibodies from ascites or cell culture supernatant Protein L is extremely useful for purification of VLκ-containing monoclonal antibodies from culture supernatant because it does not bind bovine immunoglobulins wikipedia.org
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