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nuclease

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nuclease
nuclease Biochem. (ˈnjuːklɪeɪz, -s) [a. G. nuclease, f. nucl- (in nucleoproteïd nucleoprotein): see -ase. The Ger. word was coined by Emmerich and Löw 1899, in Zeitschr. für Hygiene XXXI. 10, but in a different sense (see quot. 1902); the present meaning originated with L. Iwanoff 1903, in Zeitschr.... Oxford English Dictionary
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Nuclease
Structure specific nuclease For details see flap endonuclease. See also HindIII Ligase Micrococcal nuclease Nuclease protection assay P1 nuclease PIN domain Polymerase Serratia marcescens nuclease (benzonase wikipedia.org
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Micrococcal nuclease
Micrococcal nuclease (, S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T''', staphylococcal nuclease , spleen phosphodiesterase, Staphylococcus aureus nuclease, Staphylococcus aureus nuclease B, ribonucleate (deoxynucleate) 3'-nucleotidohydrolase) is an wikipedia.org
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Does mung bean nuclease cleave a phosphate group when it's chewing off 5' or 3' ssDNA ends? I'm looking to create blunt ends from sticky ends with mung bean nuclease for subsequent ligation. Does anyone know full mech...
Mung Bean Nuclease I. Terminally Directed Hydrolysis of Native DNA. The endonucleolytic activity of mung bean nuclease on denatured DNA also results in the production of 5’-phosphoryl-terminated products.
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Chimeric nuclease
Chimeric nucleases are an example of engineered proteins which must comprise a DNA-binding domain to give sequence specificity and a nuclease domain for Nuclease domain The nuclease domain is responsible for physical cleavage of DNA strands and may introduce either single stranded or double-stranded breaks wikipedia.org
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Serratia marcescens nuclease
Serratia marcescens nuclease (, endonuclease (Serratia marcescens), barley nuclease, plant nuclease I, nucleate endonuclease) is an enzyme. Nuclease, from Syd Labs GENIUS Nuclease, from ACROBiosystems Pierce Universal Nuclease, from Thermo Fisher Scientific TurboNuclease, from Accelagen wikipedia.org
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Function of heparin and dextran sulfate for removing proteins From this article : > The reaction was terminated and the histones, and most nonhistones, were removed by adding the nuclease-treated chromosomes to a sol...
See here. Histones are basic proteins (cationic, high pI) because they are required to interact with polyanionic DNA at physiological pH. Heparin and dextran are polyanions which form insoluble salts with the cationic histones.(Dextran is a polymer of glucose. In dextran sulphate it is derivatised w...
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Nuclease S1
Properties Aspergillus nuclease S1 is a monomeric protein of a molecular weight of 38 kilodalton. Uses Aspergillus nuclease S1 is used in the laboratory as a reagent in nuclease protection assays. wikipedia.org
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Zinc finger nuclease
Several different protein engineering techniques have been employed to improve both the activity and specificity of the nuclease domain used in ZFNs. See also Chimeric nuclease Genome editing Gene targeting Zinc finger protein Zinc finger chimera Protein engineering Zinc finger nuclease treatment of wikipedia.org
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The Nuclease A Inhibitor Represents a New Variation of the Rare PR-1 ...
Request PDF | The Nuclease A Inhibitor Represents a New Variation of the Rare PR-1 Fold | Nuclease A (NucA) from Anabaena sp. is a non-specific endonuclease able to degrade single and double ...
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Nuclease非限制性核酸内切酶-核酸消除的"终极武器"
Nuclease非限制性核酸内切酶-核酸消除的"终极武器". ——核酸消除的"终极武器". 翌圣生物研发团队利用一流的基因工程改造技术，研制而成的核酸去除的"终极武器"—非限制性核酸内切酶，既保证了产品的纯度与酶活，又确保了产品的稳定性，目标客户攘 ...
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微球菌核酸酶(Micrococcal Nuclease, MNase)(D7201M) - Beyotime
D7201M. 微球菌核酸酶 (Micrococcal Nuclease, MNase) 1600KU. 1296.00元. 碧云天生产的微球菌核酸酶 (Micrococcal Nuclease, MNase)，也被称为Micrococcal Endonuclease或S7 Nuclease，是一种来源于金黄色葡萄球菌 ( Staphylococcus aureus )的核酸内切酶，CAS号为9013-53-0，分子量约18.6kDa，在pH7.-10.0和Ca ...
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The MRE11 nuclease promotes homologous recombination not ... - Springer
Homologous recombination (HR) repairs double-strand breaks (DSBs) occurring in sister chromatids using the intact sisters as the repair template. HR is initiated by DSB resection, which generates 3′ single-strand DNA (ssDNA). RAD51 recombinase polymerizes on the ssDNA and undergoes strand exchange with intact sister chromatids, generating junction molecules (JMs). The separation of JMs ...
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An engineered Cas12i nuclease that is an efficient genome editing tool ...
Jan 8, 2024The type V-I CRISPR-Cas system is becoming increasingly more attractive for genome editing. However, natural nucleases of this system often exhibit low efficiency, limiting their application.
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Mung bean nuclease
Mung bean nuclease (Nuclease MB) is a nuclease derived from sprouts of the mung bean (Vigna radiata) that removes nucleotides in a step-wise manner from Unlike S1 Nuclease, Mung Bean Nuclease will not cleave the intact strand of nicked duplex DNA. wikipedia.org
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