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nuclease

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Nuclease - an overview | ScienceDirect Topics
Nucleases are a type of hydrolytic enzymes that cleave the phosphodiester bonds between the sugars and the phosphate moieties of nucleic acids . The cleavage reactions may occur at the end or within the strand of DNA molecules, thus DNA nucleases are usually categorized as endonucleases and exonucleases. www.sciencedirect.com
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Nuclease - Wikipedia
A nuclease (also archaically known as nucleodepolymerase or polynucleotidase) is an enzyme capable of cleaving the phosphodiester bonds that link nucleotides ... en.wikipedia.org
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Nucleases: Diversity of Structure, Function and Mechanism - PMC
Nucleases cleave the phosphodiester bonds of nucleic acids and may be endo or exo, DNases or RNases, topoisomerases, recombinases, ribozymes, ... pmc.ncbi.nlm.nih.gov
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nuclease
nuclease Biochem. (ˈnjuːklɪeɪz, -s) [a. G. nuclease, f. nucl- (in nucleoproteïd nucleoprotein): see -ase. The Ger. word was coined by Emmerich and Löw 1899, in Zeitschr. für Hygiene XXXI. 10, but in a different sense (see quot. 1902); the present meaning originated with L. Iwanoff 1903, in Zeitschr.... Oxford English Dictionary
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Nucleases (DNases and RNases) - Sigma-Aldrich
The function of nucleases (DNases and RNases) includes the enzymatic breakdown of DNA and RNA and is necessary for numerous research applications. www.sigmaaldrich.com
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Nuclease Characteristics | Thermo Fisher Scientific - US
Nuclease characteristics include grade, source, cleavage specificity, MW, pH optimum, and required cofactors. For example, RNase A cleaves 3' of ss C's and U's. www.thermofisher.com
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NUCLEASE Definition & Meaning - Merriam-Webster
The meaning of NUCLEASE is any of various enzymes that promote hydrolysis of nucleic acids. www.merriam-webster.com
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Nuclease | Description, Types, Action, Endonuclease ... - Britannica
A nuclease is any enzyme that cleaves nucleic acids by breaking phosphodiester bonds between nucleotide molecules, Nucleases act via hydrolysis, ... www.britannica.com
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Discovery of the widespread site-specific single-stranded nuclease ...
We show that the protein from Nm, renamed SsnA, is a site-specific single-stranded endonuclease capable of binding and cutting the NTS only as ... www.nature.com
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S1 Nuclease - Promega Corporation
S1 Nuclease is an endonuclease that degrades single-stranded DNA and RNA, used to remove protruding single-stranded termini from double-stranded DNA. www.promega.com
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Micrococcal nuclease
Micrococcal nuclease (, S7 Nuclease, MNase, spleen endonuclease, thermonuclease, nuclease T, micrococcal endonuclease, nuclease T''', staphylococcal nuclease , spleen phosphodiesterase, Staphylococcus aureus nuclease, Staphylococcus aureus nuclease B, ribonucleate (deoxynucleate) 3'-nucleotidohydrolase) is an wikipedia.org
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Does mung bean nuclease cleave a phosphate group when it's chewing off 5' or 3' ssDNA ends? I'm looking to create blunt ends from sticky ends with mung bean nuclease for subsequent ligation. Does anyone know full mech...
Mung Bean Nuclease I. Terminally Directed Hydrolysis of Native DNA. The endonucleolytic activity of mung bean nuclease on denatured DNA also results in the production of 5’-phosphoryl-terminated products.
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Chimeric nuclease
Chimeric nucleases are an example of engineered proteins which must comprise a DNA-binding domain to give sequence specificity and a nuclease domain for Nuclease domain The nuclease domain is responsible for physical cleavage of DNA strands and may introduce either single stranded or double-stranded breaks wikipedia.org
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Serratia marcescens nuclease
Serratia marcescens nuclease (, endonuclease (Serratia marcescens), barley nuclease, plant nuclease I, nucleate endonuclease) is an enzyme. Nuclease, from Syd Labs GENIUS Nuclease, from ACROBiosystems Pierce Universal Nuclease, from Thermo Fisher Scientific TurboNuclease, from Accelagen wikipedia.org
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Function of heparin and dextran sulfate for removing proteins From this article : > The reaction was terminated and the histones, and most nonhistones, were removed by adding the nuclease-treated chromosomes to a sol...
See here. Histones are basic proteins (cationic, high pI) because they are required to interact with polyanionic DNA at physiological pH. Heparin and dextran are polyanions which form insoluble salts with the cationic histones.(Dextran is a polymer of glucose. In dextran sulphate it is derivatised w...
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