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ligate

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1
ligate
▪ I. † ligate, a. Obs. rare—0. [ad. L. ligāt-us, pa. pple. of ligāre to bind.] ‘Bound, tied’.1604 in R. Cawdrey Table Alph.▪ II. ligate, v. Chiefly Surg. (ˈlaɪgeɪt) [f. L. ligāt-, ppl. stem of ligāre to bind.] trans. To bind with a ligature or bandage; spec. in Surg., to tie up (a bleeding artery or... Oxford English Dictionary
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Ptychatractus ligatus
Ptychatractus ligatus, common name the ligate false spindle, is a species of sea snail, a marine gastropod mollusk in the family Ptychatractidae. wikipedia.org
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perligate
† perˈligate, v. Obs. rare—0. [f. ppl. stem of med.L. perlīgāre, f. per- 2 + līgāre to bind.] To bind hard. So † perliˈgation.1623 Cockeram, Perligate. Perligation, a hard binding. Oxford English Dictionary
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Pierre Bouchet
He was the first in France to modify then use a knotted-string snare device to ligate and remove uterus and vagina polyps. His son, Claude-Antoine Bouchet, was the first, in France, to ligate external iliac artery to cure groin aneurysm. wikipedia.org
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religate
▪ I. ˈreligate, v. rare. [f. L. religāt-, ppl. stem of religāre to bind up or back: see re- and ligate v.] trans. † a. Surg. To bind up (a vein). Obs.—1 b. To bind together or unite (people). c. To constrain. Also absol. Hence ˈreligating ppl. a.1597 A. M. tr. Guillemeau's Fr. Chirurg. 17 b/2 The ne... Oxford English Dictionary
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Virama
The virāma in the sequence C1 + virāma + C2 may thus work as an invisible control character to ligate C1 and C2 in Unicode. It is also possible that the virāma does not ligate C1 and C2, leaving the full forms of C1 and C2 as they are: ka + virama + ṣa = kṣa is an example wikipedia.org
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I-III-VI semiconductors
LiGaTe2 crystals with a size up to 5 mm can be grown in three steps. The homogenization temperature is selected ca. 50 K above the melting point of LiGaTe2. wikipedia.org
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How prevalent is Taq polymerase in adding 3' A overhangs to the PCR product? I am conducting a mutagenesis on a gene in vivo of which I need to ligate into an expression vector. The primers I have designed overlap res...
You can then ligate your PCR product that has a 3' overhang.
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ACOT4
long-chain acyl-CoA synthetases, but have a unique purpose in that they generate the free acid and CoA, as opposed to long-chain acyl-CoA synthetases, which ligate wikipedia.org
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DNA ligase mechanism !enter image description here I am unable to understand what happens to the phosphates from ATP. What are they used for ?Charging 3' end or 5'end ?
When you ligate two strands of DNA you build up a phosphodiester bond.
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Promoter bashing
It is often easiest to ligate the promoter into the reporter, generate a large amount of the reporter construct using PCR or growth in bacteria, and then Ligate to reporter gene. The promoters to be assayed must be ligated to a reporter gene so that gene expression levels can be measured. wikipedia.org
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incontunded
† inconˈtunded, a. Obs. [in-3.] Not bruised or pounded.1599 A. M. tr. Gabelhouer's Bk. Physicke 51/2 Ligate a little incontundede Safferne in a little linnen cloth, soacke it 3 dayes in a little whyte wyne. Oxford English Dictionary
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Ammonium ferric citrate
The deprotonated hydroxyl group and two of the carboxylate groups ligate to the ferric center, while the third carboxylate group coordinates with the ammonium wikipedia.org
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Removing a restriction site and introducing other at its place > What would you do if you want to **remove** an EcoRI restriction site and introduce BamHI restriction site at apprx. the same location ? One of the ans...
The recognition site for EcoRI is `GAATTC`, and the enzyme cuts after the first base. See this picture from NEB: !enter image description here The overhang is: AATT, which is supplied by your oligo and fits into the overhang. If the next nucleotide whould be a `C` than the site would be recreated, s...
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基因组测序技术有哪些？
用加入一个新 nucleotide 时的 ph 值变化来测，而不是 ligate，扫描照相，切割这样的复杂过程，据说速度就快了。 质量不清楚。要从独立机构把不同技术作比对才知道。 我对现在的片子是否能把人的全部基因组扫过，是否有足够的 coverage 还有怀疑。不过，这个，理论上只是时间问题。 zhihu
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