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intron
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intron
intron Genetics. (ˈɪntrɒn) [f. intr(agenic adj. s.v. intra- + -on1: see quot. 1978.] A segment of an RNA molecule which is excised during or soon after its transcription from DNA and takes no part in forming the eventual gene; a segment of a DNA molecule which codes for this. Cf. exon2.1978, etc. [s...
Oxford English Dictionary
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Group III intron
Group III intron is a class of introns found in mRNA genes of chloroplasts in euglenid protists. Splicing is done with two transesterification reactions with a dVI bulged adenosine as initiating nucleophile; the intron is excised as a lariat.
wikipedia.org
en.wikipedia.org
Group II intron
Protein machinery is required for splicing in vivo, and long-range intron-intron and intron-exon interactions are important for splice site positioning See also
Database for bacterial group II introns
Intron
Splice site
Nuclear introns
Group I intron
Group III intron
Twintron
LtrA
References
Further
wikipedia.org
en.wikipedia.org
Are introns conserved among cells? To elaborate on the title: Among somatic, post-mitotic cells, would the same intron on a given chromosome have the same sequence among all cells descended from a progenitor cell?
Any given genomic locus (intron, exon, intergenic region) _probably_ doesn't mutate most of the time.
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Small t intron
The small T intron is an intron, that is used in some plasmid vectors, in order to induce gene expression in mammalian cells. Function
The function of this intron in the vectors is unknown, but it is theorized that it might be involved in splicing or translation efficiency.
wikipedia.org
en.wikipedia.org
What are limiting factors for intron length? For predicting genes from a sequenced genome, you need to set a maximum intron length. How long can introns get in animals? Is there some limit?
. < shows that most introns are less than about 10 kb (and personal experience in Drosophila confirms that - I've _rarely_ seen an intron bigger than about since it's nearly impossible to detect the splicing reaction, particularly if they're very large, it's not clear whether they are spliced as one BIG intron
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Intron-mediated enhancement
Intron-mediated enhancement (IME) is the ability of an intron sequence to enhance the expression of a gene containing that intron. If the intron can no longer enhance expression, then inclusion of the intron in the transcript is important, and the intron probably causes IME.
wikipedia.org
en.wikipedia.org
How to replace intron region in a plasmid? I'm considering working with the plasmid pRFHUE-eGFP and would like to replace the gpdA intron (which is the eGFP promoter region) with a promoter from another organism. Wha...
Your strategy would work, but if possible you might be better off using XmaI rather than SmaI, as the latter is a blunt-end cutting enzyme. Furthermore, your strategy would not replace the entire _gpdA_ promoter, if that's what you're after. You might wanna refer to their paper, where it's clear fro...
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Intron Depot 1
One feature of Intron Depot 1 is that all of the works are presented with a small aside from Shirow as to his thoughts on them. Intron Depot 1 in two spots in particular is quite graphic.
wikipedia.org
en.wikipedia.org
Why are minor introns bounded by nucleotides AT-AC, where T is not normally in RNA? Major intron boundary nucleotides are GU-AG. Minor intron boundary nucleotides are AT-AC. Why does the former use RNA Uracil and the ...
In RNA at the boundary of the minor intron, there is a uracil.
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Group I catalytic intron
Both intron-early and intron-late theories have found evidences in explaining the origin of group I introns. See also
Intron
Group I Intron Sequence and Structure Database
Splice site
Nuclear introns
Group II intron
Group III intron
Twintron
LtrA
Cyclic di-GMP-II
wikipedia.org
en.wikipedia.org
DNA MOLECULES COMPRISING A MODIFIED Group II INTRON
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Correlation analysis of DRB 1 gene intron 2 microsatellite polymorphism ...
Abstract: The correlation of the Gansu Alpine Merino DRB1 gene intron 2 microsatellite polymorphism and growth performance were analyzed by growth performance measurement, DNA sequencing and microsatellite DNA polymorphism detection.The results showed that the Gansu Alpine Merino DRB1 gene intron 2 microsatellite repeat unit was composed of GT and GA, which belonged to compound microsatellite ...
www.zjnyxb.cn
www.zjnyxb.cn
Exon-intron database
The Exon-Intron Database (EID) is a database of spliced mRNA sequences. See also
Alternative splicing
Exon
Intron
References
External links
Homepage
Biological databases
Gene expression
Spliceosome
RNA splicing
wikipedia.org
en.wikipedia.org
Location of TFBS in genome I have an annotated set of SNPs and I would like to explore the difference in the binding affinity of the transcription factor (TF) if I have a SNP in my locus. As my SNPs are annotated (I k...
I would link your data with the ENCODE dataset. This dataset provides locations of TFBS. It is also accessible via the UCSC genome browser. For the actual question TFBS are located pretty much everywhere, including exons (as described here), introns and of course intergenic regions (e.g. enhancers, ...
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