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chromatin

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chromatin
chromatin Biol. (ˈkrəʊmətɪn) [G. (W. Flemming 1880, in Archiv f. mikrosk. Anat. XVIII. 158), f. Gr. χρώµατ-, χρῶµα colour + -in.] A complex constituent of the cell nucleus which can be readily stained when immersed in colouring matter.1882 Gilburt in Jrnl. Quek. Cl. Ser. ii. No. 1. 32 It is proposed... Oxford English Dictionary
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Chromatin - Wikipedia
The major structures in DNA compaction: DNA, the nucleosome, the 10 nm beads on a string chromatin fibre and the metaphase chromosome. Chromatin is a complex of DNA and protein found in eukaryotic cells. The primary function is to package long DNA molecules into more compact, denser structures. This prevents the strands from becoming tangled and also plays important roles in reinforcing the ...
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DNA Packaging: Nucleosomes and Chromatin - Nature
One such enzyme, micrococcal nuclease (MNase), has the important property of preferentially cutting the linker DNA between nucleosomes well before it cuts the DNA that is wrapped around octamers ...
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Chromatin bridge
As the daughter cells exit mitosis and re-enter interphase, the chromatin bridge becomes known as an interphase bridge. Chromatin bridges may serve as a marker of cancer activity. wikipedia.org
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Regulation of chromatin structure Recently, I reviewed the different levels of chromatin structure. The primary level is nucleosomes, where DNA is bound to histones, and has structural similarity to "beads on a string...
"Are these modifications the primary regulation mechanism for chromatin structure?" Something else you can think of are the various regulatory proteins that interact with histone marks to modify chromatin.
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Chromatin variant
A chromatin variant corresponds to a section of the genome that differs in chromatin states across cell types/states within an individual (intra-individual Chromatin variants are found across the genome, inclusive of repetitive and non-repetitive DNA sequence. Chromatin variants range in sizes. wikipedia.org
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How can chromatin state be measured? I have some RNA-Seq data and I'd like to align it to the physical genome and see which sections of chromatin are geometrically open and being transcribed. The data are already sequ...
As the chromatin landscape itself is dynamic and High throughput data exploration has only begun in the last decade, consider the following tools and results You may find the following **tools helpful** : * Archalign , Publication (didn't work well during my day of trials) * Ensembl chromatin-state map
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Bivalent chromatin
The developmentally regulated process of resolving bivalent chromatin is aided by the activity of ATP-chromatin remodelers such as SWI/SNF, which hydrolyze ATP to evict Polycomb-group proteins from bivalent chromatin. wikipedia.org
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Bacterial chromatin binding data? I'm looking for data - maybe CHP^2 data that shows chromatin binding to a prokaryotic genome under some specific conditions. Can anyone point me to a source?
A large number of prokaryotes do indeed have nucleosome-like structures. The most well studied is H-NS in _E. coli_ , _Salmonella_ and some other deltaproteobacteria. H-NS like molecules have also been found in mycoplasma (Lsr2). One of its roles is to bind AT-rich DNA and silence transcription. The...
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Chromatin immunoprecipitation
Generally, native chromatin is used as starting chromatin. As histones wrap around DNA to form nucleosomes, they are naturally linked. of the target chromatin. wikipedia.org
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Why do chromosomes uncoil back to chromatin after cell divisions? At the telophase of meiotic and mitotic cell divisions, the chromosomes of daughter cells uncoil back to chromatin, but after interphase, it coils up a...
The condensed form of DNA that exists during cell division is tightly wound and therefore unavailable to the enzymes and transcription factors that interact with and read DNA. So, if chromosomes are constitutively condensed, the cell will not be able to transcribe RNA, and thus will not be able to m...
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Chromatin remodeling
Chromatin remodeling is the dynamic modification of chromatin architecture to allow access of condensed genomic DNA to the regulatory transcription machinery ATP-dependent chromatin remodeling complexes. wikipedia.org
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Chromatin Immunoprecipitation (ChIP) Assay Kit | 17-295 - Merck
17-295. Trade Name. EZ-ChIP. Description. Chromatin Immunoprecipitation (ChIP) Assay Kit. Overview. For use to enrich for specific regions of chromatin that are immunoprecipitated with a specific antibody for a chromatin protein. Works with all eukaryotic cell types. Detection of the gene or promoter of interest in immunoprecipitated chromatin ...
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Chromatin loops associated with active genes and heterochromatin shape ...
Multiscale 3D genome maps in rice. To investigate the genome architecture organization principle and its effects on transcriptional regulation in rice, we mapped active genes related chromatin architecture by capturing RNAPII and H3K4me3-associated chromatin interactions and present heterochromatin-engaged genome architecture using H3K9me2-associated interactions in young leaves of two rice ...
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Chromatin loops associated with active genes and ... - PubMed
Insight into high-resolution three-dimensional genome organization and its effect on transcription remains largely elusive in plants. Here, using a long-read ChIA-PET approach, we map H3K4me3- and RNA polymerase II (RNAPII)-associated promoter-promoter interactions and H3K9me2-marked heterochromatin …
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