mutant down but not out I am interested in a gene which is null lethal but I need to temporary induce diminished capacity. If a cell is homozygous is it possible to induce heterozygous phenotypes or a partial knockout from WT with siRNA or other molecular methods without virus vectors? I'm not interested in a medillian solution but an inducible molecular way. Thank you I would like to generate a heterozygous Sod2+/- like they do in this paper in mice and possibly human: <

Antisense, promoter replacement and when possible tetracycline allows one to titrate of gene expression <