Yes, if $w$ is not-identically-zero function with zero boundary values in some domain $D$, and $\Delta w+\lambda w=0$ holds in $D$, then you can conclude $\lambda>0$. Proof: $$0 = \int_D w(\Delta w+\lambda w) = \int_D (-|\
abla w|^2 + \lambda w^2)$$ where the first term was integrated by parts. If $\lambda\le 0$, we have a contradiction: the integral on the right is negative.