proper microscope setup is critical to help ensure optimal performance and safety when making observations or using a microscope camera proper microscope setup increases operator productivity and efficiency for a streamlined workflow for serial microscopes three of the most important and often overlooked elements of microscope setup our inner pupillary adjustment diopter and zoom parfocal ad adjustment camera and microscope part vocality adjusting the interpupillary distance is necessary to obtain proper binocular vision first place a flat sample on the stage adjust the lighting and bring the sample into focus while holding the left and right observation tubes with both hands look through the eyepieces adjust for binocular vision by moving the eyepieces together or apart until the left and right fields of view merge to form a single image for operators who wear glasses Olympus recommends using the eye shades in the normal fold down position to help prevent eyeglasses from being scratched next the stereo microscope must be properly focused to provide a clear image from low to high magnification the diopter and zoom par folk ality must be properly adjusted to provide consistently clear images through the range of magnifications the diopter adjustment is used to compensate for differences between one's eyes and is an important step in preventing eye strain that can lead to fatigue as well as eliminating the tedious task of having to refocus the microscope every time the zoom is changed to make the adjustment turn the diopter rings up the left and right eyepieces to position zero for operators with perfect 20/20 vision this is the correct setting all others require the following adjustments first place a simple large sample that will be easy to observe on the stage plate set the zoom adjustment knob for the lowest magnification and rotate the focus adjustment knob to bring the sample into focus next set the zoom adjustment knob for the highest magnification and rotate the focus adjustment knob to bring the sample into focus finally set the zoom adjustment knob for the lowest magnification and rotate the diopter adjustment ring of the left and right eyepieces to bring the sample into focus the adjustment is now complete and the image should now remain in focus from low to high zoom magnification for stereo microscopes that are used by multiple operators Olympus recommends that each operator record their diopter settings since they will vary between users for stereo microscopes equipped with the digital imaging systems proper camera par folk allottee is important to help ensure that the image viewed through the microscope and the image displayed on the monitor are in focus with each other for this procedure to work the microscope should be equipped with a camera mount that has a built-in focus adjustment first place a sample on the stage plate while looking through the eyepieces bring the image into focus using the microscope focus knob display the image on the monitor if the image on the monitor screen is blurry a par folk ality adjustment is required to adjust simply turn the focus dial on the camera adapter until the image on the monitor screen is in focus if your camera adapter has a locking mechanism lock it into place by making the three simple microscope adjustments discussed in this video users will be able to make more comfortable and efficient observations for more information on olympus industrial microscope solutions contact your local representative or visit our website at wwlp.com dot-com