Electroporation of one-cell embryo? Would electroporation be successful on a one/two-celled mouse embryo? If it would, what buffer could be used and what percentage of cells would be viable? Thank you.

The buffers are usually supplied with the electroporation instrument; at least Lonza provides buffers for different types of cells. Single cell electroporation techniques exist, but they have been mostly done on neurons.[1,2]

You can also try microinjection but it requires some practice. This study reports a transfection agent called VisuFect which can be used for zygotes as well.

I am not very sure about the success rate (AFAIK it is not that good) but I think it is anyway risky with organisms which do not produce a lot of embryos.