**exclusively** means "it is not the uncle that did it". The equivalent is ****. Yes, it comes from question "Isn't it —?" then becomes to mean "How could it not —". And in formal saying you _cannot_ drop the question particle .
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By the way, if written, the casual is certainly ambiguous even to Japanese, but their difference is clear in speech.
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 is negative "did not", and the second "surely did" (=).