No, it is not unique at least starting from $n=3,$ so an $8 \times 8$ board. Note that two L trominoes can combine to make a $2 \times 3$ rectangle. This rectangle can have the pieces placed two ways. Now let the removed square be a corner. Put one L next to it to make a $2 \times 2$ square. You can tile the rest of the board with five $2 \times 6$ rectangles as shown below. Each $2 \times 6$ can be tiled in four ways, so this gives $1024$ ways to tile the square. I believe the $n=2$ case is unique but have not done a careful search to prove it.
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