"Would that affect calculus?" Of course not, and calculus in its present form would even remain a perfect tool for describing the macroscopic world. Note that, e.g,. we already _know_ that matter comes in the form of "little balls", but nevertheless we treat it as a homogeneous "glue" when we do elasticity or hydrodynamics.
On the other hand it might be the case that some sort of new mathematics would be needed to describe and understand better time and space in its ultimate fine grained structure.