qTERT-pcr like qRT-pcr Is it possible to use a biologically active Telemorease Elongation Reverse Transcriptase (TERT) in the place of the Reverse Transcriptase (RT) for quantitative Reverse Transcriptase pcr (qRT-pcr)? Yes I do know that it would seemingly have no advantages. What kind of reverse transcriptase is used in qRT-pcr is there a standard like taq dna pol is used for qpcr?

> What kind of reverse transcriptase is used in qRT-pcr is there a standard like taq dna pol is used for qPCR

M-MuLV (Monoley murine leukemia virus) reverse transcriptase

Some RT enzymes are engineered to remove the RNAseH domain so that template RNA persists for more rounds of RT.

> Is it possible to use a biologically active Telemorease Elongation Reverse Transcriptase (TERT) in the place of the Reverse Transcriptase

Perhaps it can catalyse an RT reaction in-vitro. But commercial enzymes need to be efficient and I think using TERT won't be that advantageous.