Do disaccharidases require a cofactor or coenzyme to function? I'm doing research on lactose intolerance and am curious if disaccharidases (enzymes that break down disaccharides) require a cofactor or coenzyme to function? Reviews or references would be greatly appreciated.

It seems that beta-glucosidases do not require co-factors but some alpha-glucosidases are co-factor dependent.

Some bacterial alpha-glucosidases (GH4 family) require NAD+ to function (Hall et al., 2009). _Thermotoga maritama_ alpha-glucosidase (GH4 family) requires Mn2+, in addition to NAD+ to function (Lodge et al., 2003). _Ferroplasma acidophilum_ alpha-glucosidase seems to contain iron but its role as a co-factor is not known (Ferrer et al., 2005).

For more information on alpha-glucosidases see BRENDA.