close to the home of my parents-in-law there is a pond and of course this is a good opportunity to pick up some water samples this is the water sample I also included a water plant and I think they're gonna be plenty of microorganisms are growing on the plant I think that should do it time to pack up and go back home to have a cup of coffee well for me microscope is not only about the laboratory work or the observing of specimens but it's also about observing nature so that is one of the things that I like about this hobby well that was a pretty rapid jump from the pond directly to my lab the plant material is quite soft this is probably because it has already started to decay and I expect plenty of microorganisms growing on these leaves a little bit of water I have to add here and of course everything goes under the microscope oh wow well that is something that I have not seen before at least not in that amount it's a whole colony of work jelly that's interesting warty jelly are ciliates they're stocked ciliates they grow on a little stalk and they retract very rapidly as a matter of fact so quickly that it's impossible to capture on camera so you need a high-speed camera to do that there are also plenty of other algae floating around this is now a dark field in which it looks much nicer now you can also see that the leaf on the bottom part is actually green and the Vorta chale they appear white and get a higher magnification algae is filamentous algae in the middle the resolution is not quite quite well because it was difficult to focus because the was quite a lot of water on on the slide there were also other algae floating around in small colonies and if you look very carefully you should be able to say that the cells have little lines extending from them these are a flood gelee and they are used for moving and you sometimes it was even possible to see them a bit and move they are green so of course as they do photosynthesis yes now you can see the flagella much better and you can see that they're extending outwards and that they're moving in the phase contrast microscope I think it would be much easier to see unfortunately I don't have a phase contrast microscope and another colony of several of them aggregated and I don't know how they stick together it could be some kind of a gel you almost can see that a little bit so it seems to be a layer of gel gel like layer around the individual colonies that makes them stick together also very nice in the background you now can pick a size comparison and you see again imported gelee and you can also see that the vorticella they also have cilia around it's one part this is yeah this seems to be I don't know if it's belongs to the genus Paramecium or not I think it's also a cell yet and also here you can see the little hair on the surface that are beating and moving and they are green because they have actually also eat some algae but if it's Paramecium if that is a were the case then Paramecium itself is actually not photosynthetic but it's a heterotroph hearing now you see this small cilia quite well and because the you're a little bit pressed flat because they're squeezed between the slide and the cover glass and this also makes it a little bit easier to see because they cannot swim in and out of focus just like the other much smaller microorganisms can do over here in the centre right the object gliding towards the bottom that is a diatom and they have a silica they have a silica shell this Stentor it looks like a trumpet it's also ciliate and there are also sub cilia on the right side and you can see that some of the debris in particles are sucked into the mouth off of stendra now you can see the cilia quite well they're beating quite rapidly here too I think it would be nice to have some kind of a high-speed camera to be able to capture the movement but I've also heard that maybe by adding a little bit of of glycerin or glycerol then you should also be able to slow down the beating of the cilia as well but have not tried this at a forty M with a 4x objective you see all the algae floating around quite a lot and I caught a little water flea as well and I did not want to squash it with my cover glass so I placed a cover glass left and right as a spacer because the cover glass that I put now on top across should be lifted up a little bit because I did not want to squeeze the little little crustacean and add it again a little bit of some water and this limited the movement of the water flea and the two large dark things extending towards the top right these seem to be eggs but I'm going to be switching to dark field right now because you see much better this way yes that's the dark field image and now you can see the egg pouches maybe I think this is about 2 X me but your could be actually like an accumulation of several eggs I'm not quite sure about that you know so that is the view from from the side this is a view from the top or from the bottom yeah the line on the left side that is the edge of the cover glass and you can also actually see the movement of the organs inside this crustacean crustaceans are little crabs and there are I think after it's or 600 of these species and they move in a very jerking way on the right side you see maybe a little red dot that is the eye spot of the organism and some of them have to have one I spot and they're able to send light well let's turn it off now I think this was a quite a nice and interesting microscopy session pond water is of course understandably one of the more favorite samples so to be observed under the microscopes because there simply is so much life diversity in a simple drop of water but at the same time I'd like to tell you a little bit that it's maybe not the water itself that is so interesting but rather the organisms that are growing on plant material because many of the organisms they you have to attach to a surface or they're using the plant material as a source of food if the decomposing it for example or they're simply attached to the surface so that they're not washed away so if you want to try some across copy of pond water samples always put a small amount of plant material take a lot more always a small amount of plant material to observe under the microscope well as always I hope that you will enjoy this video please subscribe to the video if you liked it and as always I'd like to wish you happy microscopy you